Autograft of Vitrified Mouse Ovaries Using Ethylene Glycol as Cryoprotectant.
نویسندگان
چکیده
منابع مشابه
the vitrification of metaphase ii mouse oocyte using ethylene glycol as cryoprotectant
introduction: the purpose of this study was to investigate the effect of vitrification procedure on the survival, fertilization, development and ultrastructure of metaphase (mii) mouse oocyte. materials and methods: female nmri mice 6-10 weeks old were superovulated using ip injection of 10 iu hmg and 10 iu hcg. ovulated oocyte were collected from the ampullary portion of the oviducts at 12-13 ...
متن کاملVitrification of mouse ovaries using ethylene glycol and DMSO as cryoprotectants: histopathological evaluation
Ten, 4to 6-week-old BALB/c mice were randomly assigned to either control (non-vitrified, n = 5) or treatment (vitrified, n = 5) groups. Ovaries in the vitrified group were frozen sequentially by immersion into two vitrification solutions VS1: 10% ethylene glycol (EG) + 10% DMSO in holding medium (TCM-199 + 20% FBS) and VS2: 20% EG + 20% DMSO in holding medium. After thawing at 37 °C in 1.0 M su...
متن کاملIn vitro viability of mouse oocytes vitrified in an ethylene glycol-based solution.
Ovulated mouse oocytes denuded of their cumulus cells, were vitrified in a solution containing 7 M ethylene glycol as the sole cryoprotectant using one or two steps of exposure before vitrification and were diluted in 1 M sucrose solution in 5 or 10 min after warming. The results proved that the viability of oocytes are detrimentally affected by exposure to the vitrification solution even witho...
متن کاملMeasurement of the apparent diffusivity of ethylene glycol in mouse ovaries through rapid MRI and theoretical investigation of cryoprotectant perfusion procedures.
Successful organ cryopreservation will significantly benefit human health and biomedical research. One of the major challenges to this accomplishment is the need for optimization of cryoprotectant agent (CPA) perfusion procedures that involve highly complicated mass transfer processes in organs. The diffusivity of CPA is of critical importance for designing perfusion procedures to minimize the ...
متن کاملNormal gestations and live births after orthotopic autograft of vitrified-warmed hemi-ovaries into ewes.
BACKGROUND The aim of this study was to evaluate the long-term outcome of autotransplantation of vitrified warmed hemi-ovaries into ewes. METHODS Six hemi-ovaries from six ewes aged 6 to 12 months were vitrified. After dissection of the medulla, the hemi-ovarian cortex was stored at -196 degrees C in liquid nitrogen. Four to six weeks after the first laparotomy, the left ovary was removed and...
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ژورنال
عنوان ژورنال: Experimental Animals
سال: 2002
ISSN: 1341-1357,1881-7122
DOI: 10.1538/expanim.51.509